コモンマーモセットにおけるZFXおよびZFY遺伝子を用いた性別判定法
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概要
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We investigated sex determination via the ZFX and ZFY genes using PCR-RFLP in the common marmoset. We designed a novel primer set to detect ZFX and ZFY. A 483-bp band from the ZFX gene and a 471-bp band from the ZFY gene were amplified. Sequencing data of the products amplified from ZFX and ZFY showed the recognition sites of two restriction enzymes, DdeI and MseI, respectively. After digestion of the products using each enzyme, we found that the band patterns between females and males were different. PCR-based sex identification might provide a tool for further breeding studies and experimental embryological studies using marmosets.
- 2011-07-01
著者
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Katoh Hideki
Experimental Animal Center Hamamatsu University School Of Medicine
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Takabayashi Shuji
Experimental Animals Inst. Hamamatsu Univ. School Of Medicine
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Takabayashi Shuji
Experimental Animals Institute Hamamatsu University School Of Medicine
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高林 秀次
Experimental Animals Institute, Hamamatsu University School of Medicine
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加藤 秀樹
Experimental Animals Institute, Hamamatsu University School of Medicine
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- コモンマーモセットにおけるZFXおよびZFY遺伝子を用いた性別判定法