Analysis of T Cell Responses to a Superantigen, Staphylococcal Enterotoxin-B

概要

論文の詳細を見る
T cell subsets that responded to a superantigen, staphylococcal enterotoxin-B (SEB), were analyzed using responder cells stained with carboxyfluorescein diacetate succinimidyl ester (CFSE). It was shown that T cells dividing 5 times represented the major population 4 days after stimulation with SEB. When the proportion of each T cell subset was analyzed sequentially, Vβ8+ T cells that represented the SEB-responding repertoire increased and reached more than 20% of viable cells 4 days after culture with SEB. Both CD4+ and CD8+ T cells in the Vβ8+ population showed similar increases. By contrast, the proportion of SEB-non-reactive Vβ6+ Tcells showed no considerable increases. The Vβ6+ T cells exhibited prominent apoptosis from an early phase in the culture, whereas Vβ8+ T cells showed activation-induced cell death at the later stage. However, the proportion of dividing cells (CFSElow) in living cells increased substantially in every T cell subset, including NK-T cells, suggesting that a bystander cell proliferation effect was involved. T cells stimulated with SEB produced both Th1 and Th2 type cytokines, although production of each cytokine followed different time courses.
日本リンパ網内系学会の論文
2004-04-01