Detection of Sugar Accumulation and Expression Levels of Correlative Key Enzymes in Winter Wheat (Triticum aestivum) at Low Temperatures
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概要
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Carbohydrate accumulation is common in frost-resistant plants, and many enzymes participate in this process. The sugar content and expression levels of metabolic enzymes related to sugar biosynthesis in response to drops in temperature were measured in two cultivars of winter wheat (Triticum aestivum) with different cold tolerances. The results indicate that the two cultivars examined, Dongnongdongmai 1 and Jimai 22, accumulated high levels of carbohydrate before November 4 (above 0 °C), and that accumulation decreased as temperatures fell. However, this decrease was more modest in Dongnongdongmai 1, which had a higher sugar content. Sucrose and fructose were the main soluble sugars, indicating an important role in freezing tolerance. Gene expression studies revealed that expression of the genes encoding chloroplastic enzymes was significantly upregulated in the tillering nodes. Expression upregulation of TaSS and TaTPT may be helpful for sugar accumulation before November 4.
- 2011-04-23
著者
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Zeng Yan
College Of Chemistry Central China Normal University
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Yu Jing
College Of Life Science Northeast Agricultural University
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Zeng Yan
College Of Life Science Northeast Agricultural University
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CANG Jing
College of Life Science, Northeast Agricultural University
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LIU Lijie
College of Life Science, Northeast Agricultural University
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MU Yongchao
College of Life Science, Northeast Agricultural University
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WANG Junhong
College of Life Science, Northeast Agricultural University
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ZHANG Da
College of Life Science, Northeast Agricultural University
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Mu Yongchao
College Of Life Science Northeast Agricultural University
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Wang Junhong
College Of Life Science Northeast Agricultural University
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Cang Jing
College Of Life Science Northeast Agricultural University
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Zhang Da
College Of Life Science Northeast Agricultural University
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Liu Lijie
College Of Life Science Northeast Agricultural University
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- Detection of Sugar Accumulation and Expression Levels of Correlative Key Enzymes in Winter Wheat (Triticum aestivum) at Low Temperatures
- Effects of Exogenous Abscisic Acid on Carbohydrate Metabolism and the Expression Levels of Correlative Key Enzymes in Winter Wheat under Low Temperature