Characterization of Chitosanase of a Deep Biosphere Bacillus Strain
スポンサーリンク
概要
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A chitosanase of deep-biosphere Bacillus thuringiensis strain JAM-GG01 was purified. The optimal pH and temperature for the purified enzyme (Cho-GG) were about pH 6 and 60 °C, but Cho-GG was unexpectedly unstable under incubation at over 40 °C. This discrepancy between higher activity and lower stability in the same range of temperature was abolished by the addition of reaction products, chitotriose and chitotetraose. The Cho-GG gene was amplified by PCR and sequenced. The deduced amino acid sequence of Cho-GG showed more than 98% identity to those of other Bacillus enzymes belonging to GH family 8. Although Cho-GG did not show the definite characteristics of a sub-seafloor ectoenzyme, the thermal stability of many chitosanases of B. turingienesis and other related strains can be improved by adding chitotriose or chitotetraose.
- 2011-04-23
著者
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Horikoshi Koki
Institute Of Biogeosciences Japan Agency For Marine-earth Science And Technology (jamstec)
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KOBAYASHI Tohru
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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KOIDE Osamu
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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DEGUCHI Shigeru
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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Koide Osamu
Institute Of Biogeosciences Japan Agency For Marine-earth Science And Technology (jamstec)
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Deguchi Shigeru
Institute Of Biogeosciences Japan Agency For Marine-earth Science And Technology (jamstec)
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Kobayashi Tohru
Institute Of Biogeosciences Japan Agency For Marine-earth Science And Technology (jamstec)
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Sikder Md.
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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HORIKOSHI Koki
Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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