Establishment and Evaluation of an in vitro M Cell Model using C2BBe1 Cells and Raji Cells
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概要
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In vitro M cell models, consisting of co-cultures of Caco-2 cells and lymphoid cells, were developed and examined to observe bacterial transport. However, under our experimental conditions, the differentiation of Caco-2 cells into M cell-like cells could not be induced efficiently. To obtain a functionally stable M cell model based on human cells, C2BBe1 cells were screened and co-cultured with human Raji cells. In our co-cultures, increased sialyl Lewis A antigen expression and decreased Ulex europeaus agglutinin 1 binding were observed. Regarding the functional properties of the model, microsphere and lactic acid bacteria transport across the C2BBe1 co-cultures were increased compared with the levels seen in monocultures. The C2BBe1 monolayers that were co-cultured with Raji cells exhibited some M cell features; therefore, we consider our M cell model to be useful for investigating the interactions of bacteria with M cells.
- 2011-04-01
著者
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Igimi Shizunobu
United Graduate School Of Veterinary Sciences Gifu University
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MASUDA Kazuya
United Graduate School of Veterinary Sciences, Gifu University
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KAJIKAWA Akinobu
Department of Food, Bioprocessing, Nutrition Sciences, North Carolina State University
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Kajikawa Akinobu
Department Of Food Bioprocessing Nutrition Sciences North Carolina State University
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Masuda Kazuya
United Graduate School Of Veterinary Sciences Gifu University
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Kajikawa Akinobu
Department Of Food Bioprocessing & Nutrition Sciences North Carolina State University
関連論文
- Establishment and Evaluation of an in vitro M Cell Model using C2BBe1 Cells and Raji Cells
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