Chromosome banding and FISH with rDNA in diploid and tetraploid loach, Misgurnus anguillicaudatus

概要

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The chromosomes of diploid and tetraploid loach, Misgurnus anguillicaudatus, were analyzed by staining with Ag, Chromomycin A3 (CMA3)/ Distamycin A (DA), and DA/4',6-diamidino-2-phenylindole (DAPI), and fluorescence in situ hybridization (FISH) with 5.8S + 28S rDNA as a probe. Nucleolus organizing regions (NORs) were mapped to the telomeric region of the short arms of the largest (first) metacentric chromosome pair in diploid loach with 2n=50 and the homologous quartet in tetraploid loach with 4n=100. The NORs were positive at the same region of the first metacentric chromosome for Ag- and CMA3/DA-stainings, but negative for DA/DAPI-staining. Four signals at the homologues within the same quartet suggest the duplication of entire genome from diploid to tetraploid status. However, size difference was detected among rDNA signals by FISH and CMA3-banding.
Springerの論文
2010-11-05