簡易炭酸ガス培養器を用いたエジプト水牛からの体外受精胚作出の新しい試み
スポンサーリンク
概要
- 論文の詳細を見る
Buffalo ovaries were collected from a slaughter house and cumulus oocyte complexes were aspirated from follicles 2-6 mm in diameter. A portable CO2 incubator and a CR1aa medium were used for in vitro production of embryos. In the first experiment, numbers of follicles and oocytes per ovary were counted, and classified according to the appearance of the cumulus cells. In the second experiment, in vitro fertilization and cultivation of buffalo oocytes matured in vitro were carried out using semen from three different bulls, which had different sperm motilities (Bull 1: 20%, Bull 2: 60% and Bull 3: 15%, respectively). In the third experiment, the cleavage and transferable embryos of fertilized oocytes from denuded oocytes, and oocytes with intact cumulus cell layers were compared. The percentages of good, fair and poor quality oocytes were 60.9%, 17.8% and 21.4%, respectively. The rates of cleavage and transferable embryos were significantly higher after incubation with semen from Bull 2 (81% and 40.2%) than those after incubation with semen from Bulls 1 (52% and 13.5%, p<0.05 and p<0.01) and 3 (15.8% and 0%, p<0.01). The rates of cleavage and transferable embryos were significantly higher (p<0.01) for oocytes with an intact cumulus than for oocytes without cumulus cells at fertilization.
- 日本繁殖生物学会の論文
- 1999-10-01
著者
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Otoi T
Yamaguchi Univ. Yamaguchi Jpn
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Murakami Masao
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
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Otoi Takeshige
United Graduate School of Veterinary Sciences, Yamaguchi University, Yamaguchi 753-8515, Japan
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Suzuki Tatsuyuki
United Graduate School Of Veterinary Science Yamaguchi University
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Murakami M
Yamaguchi Univ. Yamaguchi Jpn
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KANDIL Omaima
Dept. of Animal Reproduction and Artificial Insemination, National Research Centre
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ABDOON Ahmed
Dept. of Animal Reproduction and Artificial Insemination, National Research Centre
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Suzuki T
Yamaguchi Univ. Yamaguchi Jpn
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Otoi Takeshige
United Graduate School Of Veterinary Sci. Yamaguchi Univ.
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Abdoon Ahmed
Dept. Of Animal Reproduction And Artificial Insemination National Research Centre
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Kandil Omaima
Dept. Of Animal Reproduction And Artificial Insemination National Research Centre
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村上 正夫
United Graduate School of Veterinary Science, Yamaguchi University
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- 簡易炭酸ガス培養器を用いたエジプト水牛からの体外受精胚作出の新しい試み
- Superovulation in Beef Cows and Heifers with a Single Injection of FSH dissolved in Polyvinylpyrrolidone
- Effects of Exposure to Zearalenone on Porcine Oocytes and Sperm During Maturation and Fertilization In Vitro
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