Sal k 4, a New Allergen of Salsola kali, Is Profilin : A Predictive Value of Conserved Conformational Regions in Cross-Reactivity with Other Plant-Derived Profilins
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概要
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The aim of this study was to investigate a new allergen of Salsola kali, Sal k 4, and to investigate the predictive value of the conserved conformational regions in cross-reactivity with other plant-derived profilins. The Sal k 4-coding sequence was cloned, expressed, and purified by one-step Ni2+ affinity chromatography to recover high-purity target protein. We assessed cross-reactivity and predicted conserved conformational regions among rSal k 4 and other plant-derived profilins. Immunodetection and inhibition assays using 30 individual sera from S. kali allergic patients indicated that purified rSal k 4 might be the same as that in the crude extract. The results of inhibition assays among rSal k 4 and other plant-derived profilins were in accordance with the homology of the predicted conserved conformational regions. Amino acid sequence homology analysis showed that a high degree of IgE cross-reactivity among plant-derived profilins might depend on the predicted conserved conformational regions.
- 社団法人 日本農芸化学会の論文
- 2010-07-23
著者
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Assarehzadegan Mohammad
Immunology Dep. Fac. Of Medicine Ahvaz Jundishapur Univ. Of Medical Sciences
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Amini Akram
Immunobiochemistry Laboratory Immunology Research Center Avicenna Research Institute
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SANKIAN Mojtaba
Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences
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TEHRANI Mohsen
Immunobiochemistry Laboratory, Immunology Research Center, Avicenna Research Institute
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JABBARI Farahzad
Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences
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VARASTEH AbdolReza
Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences
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Tehrani Mohsen
Immunobiochemistry Laboratory Immunology Research Center Avicenna Research Institute
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Jabbari Farahzad
Immunology Research Center Avicenna Research Institute Mashhad University Of Medical Sciences
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Varasteh Abdolreza
Immunology Research Center Avicenna Research Institute Mashhad University Of Medical Sciences
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Sankian Mojtaba
Immunology Research Center Avicenna Research Institute Mashhad University Of Medical Sciences
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Sankian Mojtaba
Immunobiochemistry Lab Immunology Research Center Avicenna Research Institute Mashhad University Of
関連論文
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- Sal k 4, a New Allergen of Salsola kali, Is Profilin : A Predictive Value of Conserved Conformational Regions in Cross-Reactivity with Other Plant-Derived Profilins
- Allergy to Salsola Kali in a Salsola Incanescens-rich Area : Role of Extensive Cross Allergenicity
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