Identification and Characterization of the Genes Responsible for the Production of the Cyclic Lipopeptide Arthrofactin by Pseudomonas sp. MIS38
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概要
- 論文の詳細を見る
Pseudomonas sp. MIS38 produces an effective biosurfactant named arthrofactin, which is a cyclic lipopeptide synthesized by a mega complex composed of three nonribosomal peptide synthetases. In order to gain insight into the control mechanism of arthrofactin production, a Tn5 mutant library was constructed and screened for arthrofactin-deficient mutants. Along with a number of mutations that occurred in the arthrofactin synthetase operon, three other mutants harbored distinct Tn5 insertions in the genes encoding SyrF-like protein (arfF), heat shock protein (htpG), and (p)ppGpp synthetase/hydrolase (spoT). Epistasis analyses revealed that spoT functions early in the arthrofactin production pathway. We also found that spoT affects MIS38 swarming, biofilm formation, and the cell morphology.
- 社団法人 日本農芸化学会の論文
- 2010-05-23
著者
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Washio Kenji
Division Of Biosphere Science Graduate School Of Environmental Science Hokkaido University
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LIM Siew
Department of Molecular and Cell Biology, University of California
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ROONGSAWANG Niran
BIOTEC Central Research Unit, National Center for Genetic Engineering and Biotechnology
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MORIKAWA Masaaki
Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University
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Lim Siew
Department Of Molecular And Cell Biology University Of California
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Washio Kenji
Div. Of Biosphere Sci. Graduate School Of Environmental Sci. Hokkaido Univ.
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Morikawa Masaaki
Division Of Biosphere Science Graduate School Of Environmental Science Hokkaido University
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Roongsawang Niran
Biotec Central Research Unit National Center For Genetic Engineering And Biotechnology
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Lim Siew
Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology, Toyohashi, Aichi 441-8580, Japan
関連論文
- The Role of Urease Activity on Biofilm Formation by Staphylococcus sp. T-02 Isolated from the Toilet Bowl
- Identification and Characterization of the Genes Responsible for the Production of the Cyclic Lipopeptide Arthrofactin by Pseudomonas sp. MIS38
- Functional Analysis of A Pyoverdine Synthetase from Pseudomonas sp. MIS38
- Functional Analysis of A Pyoverdine Synthetase from Pseudomonas sp. MIS38
- The Role of Urease Activity on Biofilm Formation by Staphylococcus sp. T-02 Isolated from the Toilet Bowl
- A truncated form of spoT, including the ACT domain, inhibits the production of cyclic lipopeptide arthrofactin, and is associated with moderate elevation of guanosine 3',5'-bispyrophosphate level in Pseudomonas sp. MIS38
- Beneficial Biofilm Formation by Industrial Bacteria Bacillus subtilis and Related Species
- A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38
- A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3',5'-Bispyrophosphate Level in Pseudomonas sp. MIS38
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