A Novel Catalytic Ability of γ-Glutamylcysteine Synthetase of Escherichia coli and Its Application in Theanine Production

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概要

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• γ-Glutamylcysteine synthetase (γGCS, EC 6.3.2.2) catalyzes the formation of γ-glutamylcysteine from L-glutamic acid (Glu) and L-cysteine (Cys) in an ATP-dependent manner. While γGCS can use various amino acids as substrate, little is known about whether it can use non-amino acid compounds in place of Cys. We determined that γGCS from Escherichia coli has the ability to combine Glu and amines to form γ-glutamylamides. The reaction rate depended on the length of the methylene chain of the amines in the following order: n-propylamine > butylamine > ethylamine >> methylamine. The optimal pH for the reaction was narrower and more alkaline than for the reaction with an amino acid. The newly found catalytic ability of γGCS was used in the production of theanine (γ-glutamylethylamine). The resting cells of E. coli expressing γGCS, in which ATP was regenerated through glycolysis, synthesized 12.1 mM theanine (18 h) from 429 mM ethylamine.

• 社団法人 日本農芸化学会の論文
• 2009-12-23

著者

• Kakita Shingo

  Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd

• Kakita Shingo

  Tokyo Research Park Kyowa Hakko Kirin Co. Ltd.

• Miyake Koichiro

  Technical Research Laboratories Kyowa Hakko Bio Co. Ltd.

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