Expression Profiling of a Novel Calcium-Dependent Protein Kinase Gene, LeCPK2, from Tomato (Solanum lycopersicum) under Heat and Pathogen-Related Hormones

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概要

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• A full-length cDNA LeCPK2 (GenBank GQ205414) from tomato (Solanum lycopersicum) encoding a calcium-dependent protein kinase (CDPK) was cloned by in silico cloning using NtCPK5 (AY971376) as a virtual probe. The deduced amino acid sequence of LeCPK2 contained the kinase, autoinhibitory, and calmodulin-like domains typical of CDPKs. Expression profiling indicated that LeCPK2 expressed predominantly in flowers and responded divergently to heat and cold stress, in which obvious mRNA accumulation was detected at 4 h under 42 °C stress, but no change in LeCPK2 mRNA levels was observed in 6 h at 4 °C. Mechanical wounding and phytohormones including ethylene, methyl jasmonate, and salicylic acid were also observed to arouse the expression of LeCPK2 in a similar pattern. mRNA accumulation was enhanced at 30 min and reached a maximum at 3 h, followed by a decrease to the normal level. All the results suggest that LeCPK2 is a novel versatile isoform of tomato CDPKs.

• 2009-11-23

著者

• Chang Wen-Jun

  Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science

• Su Huo-Sheng

  Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences

• Li Wei-Jing

  Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science

• ZHANG Zhi-Li

  Hainan Academy of Agricultural Sciences

• Zhang Zhi-li

  Hainan Acad. Of Agricultural Sciences

• Chang Wen-jun

  Key Lab. Of Rubber Biology Ministry Of Agriculture/state Key Lab. Breeding Base Of Cultivation And P

• Li Wei

  Institute Of Thin Films And Nanomaterials Department Of Mathematics And Physics Wuyi University

• Su Huo-sheng

  Sugarcane Research Institute Yunnan Academy Of Agricultural Sciences

• Li Wei

  Institute Of Tropical Bioscience And Biotechnology Chinese Academy Of Tropical Agricultural Sciences

• Li Wei

  Institute Of Plant Protection Jiangsu Academy Of Agricultural Sciences

関連論文

• Expression Profiling of a Novel Calcium-Dependent Protein Kinase Gene, LeCPK2, from Tomato (Solanum lycopersicum) under Heat and Pathogen-Related Hormones
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• Molecular Characterization of HbCDPK1, an Ethephon-Induced Calcium-Dependent Protein Kinase Gene of Hevea brasiliensis
• Expression Profiling of a Novel Calcium-Dependent Protein Kinase Gene, LeCPK2, from Tomato (Solanum lycopersicum) under Heat and Pathogen-Related Hormones
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