Suppressed-Priming PCR, a Novel Concept of DNA Quantification Based on PCR Kinetics

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概要

• 論文の詳細を見る

• The concept of an unbiased DNA quantification method is proposed based on PCR kinetics. A set of PCRs was performed with a dilution series of primers, which differently limited DNA amplification. The difference in amplification efficiency between the DNA templates reflected the estimates of their relative amounts. This concept might serve as a theoretical base for further development of accurate and robust PCR-based quantification methods.

• 社団法人 日本農芸化学会の論文
• 2009-09-23

著者

• TAKEMOTO Shuhei

  National Institute for Agro-Environmental Sciences

• OKADA Hiroaki

  National Institute for Agro-Environmental Sciences

• Takemoto Shuhei

  National Inst. For Agro-environmental Sciences

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