Effect of a Novel Recombinant Protein of FibronectinIII7-10/Cadherin 11 EC1-2 on Osteoblastic Adhesion and Differentiation
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概要
- 論文の詳細を見る
The limitations of specific adhesion and osteoblastic differentiation are current problems in bone tissue engineering. The aim of this study was to investigate the effect of a novel recombinant protein of fibronectin module III7-10/cadherin 11 EC1-2 (rFN/CDH) on cell adhesion and differentiation. Gene coding rFN/CDH was engineered by a homology modeling strategy, and an expression plasmid was constructed by standard DNA techniques. The rFN/CDH protein was expressed in Rosetta-gami (DE3), an improved Escherichia coli system. MC3T3-E1 cell centrifugal adhesive assay indicated that the adhesive capacity of rFN/CDH was significantly improved. Quantitative analysis of two osteogenic markers, osteocalcin mRNA expression and alkaline phosphatase activity, indicated that they were further up-regulated when human mesenchymal stem cells were cultured for 7–10 d on rFN/CDH pre-coated surfaces. These results suggest that rFN/CDH possesses an enhanced dual biofunctionality in osteoblastic adhesion and differentiation, and a promising application can be expected in biomimetic strategies and biomaterial development.
- 社団法人 日本農芸化学会の論文
- 2009-09-23
著者
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ZHANG Yuan
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi Universi
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Zhu Jie
Department of Bioinformatics, Medical Research Institute, Tokyo Medical and Dental Univerisity
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Zhang Yuan
Department Of Orthopedics Xinqiao Hospital Third Military Medical University
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Xiang Qiang
Emergency Department Southwest Hospital Third Military Medical University
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ZHOU Yue
Department of Orthopedics, Xinqiao Hospital, Third Military Medical University
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DONG Shiwu
Department of Anatomy, Third Military Medical University
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LI Changqing
Department of Orthopedics, Xinqiao Hospital, Third Military Medical University
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Dong Shiwu
Department Of Anatomy Third Military Medical University
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Zhou Yue
Department Of Orthopedics Xinqiao Hospital Third Military Medical University
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Li Changqing
Department Of Orthopedics Xinqiao Hospital Third Military Medical University
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Zhu Jie
Department Of Neurology Daping Hospital Third Military Medical University
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Zhang Yuan
Department Of Applied Chemistry And Chemical Engineering Faculty Of Engineering Yamaguchi University
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Zhu Jie
Department Of Bioinformatics Medical Research Institute Tokyo Medical And Dental Univerisity
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Zhou Yue
Department Of Orthopaedic Surgery Affiliated Xinqiao Hospital The Third Military Medical University
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