Cloning and Characterization of Flagellin Genes and Identification of Flagellin Glycosylation from Thermophilic Bacillus Species
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概要
- 論文の詳細を見る
Flagellin glycosylation was identified in Bacillus sp. PS3 and Geobacillus stearothermophilus. In vivo complementation showed that these flagellin genes did not restore the motility of a Bacillus subtilis flagellin mutant, whereas the genes encoding non-glycosylated flagellin from Geobacillus kaustophilus and Bacillus sp. Kps3 restored motility. Moreover, four types of flagellins expressed in B. subtilis were not glycosylated. We speculate that glycosylation is required for flagellar filament assembly of these bacilli.
- 2009-06-23
著者
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HAYAKAWA Jumpei
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
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ISHIZUKA Morio
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
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Ishizuka Morio
Department Of Applied Chemistry Faculty Of Science And Engineering Chuo University
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Hayakawa Jumpei
Department Of Applied Chemistry Faculty Of Science And Engineering Chuo University
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KONDOH Yoshihide
Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University
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Kondoh Yoshihide
Department Of Applied Chemistry Faculty Of Science And Engineering Chuo University
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Ishizuka Morio
Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Chuo Univ.
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Hayakawa Jumpei
Dep. Of Applied Chemistry Fac. Of Sci. And Engineering Chuo Univ.
関連論文
- A Group I Self-Splicing Intron in the Flagellin Gene of the Thermophilic Bacterium Geobacillus stearothermophilus
- Screening of a Mutant Plasmid with High Expression Efficiency of GC-Rich leuB Gene of an Extreme Thermophile, Thermus thermophilus, in Escherichia coli
- Amino Acid Substitutions and Intragenic Duplications of Bacillus sp. PS3 Flagellin Cause Complementation of the Bacillus subtilis Flagellin Deletion Mutant
- Cloning and Characterization of Flagellin Genes and Identification of Flagellin Glycosylation from Thermophilic Bacillus Species
- A Group I Self-Splicing Intron in the Flagellin Gene of the Thermophilic Bacterium Geobacillus stearothermophilus
- Temperature-Dependent Self-Splicing Group I Introns in the Flagellin Genes of the Thermophilic Bacillus Species
- Proteomic Analysis of the Streptomyces griseus Ribosomal Fraction