Cloning and Characterization of a β-1, 4-Mannanase 5C Possessing a Family 27 Carbohydrate-Binding Module from a Marine Bacterium, Vibrio sp. Strain MA-138

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概要

• 論文の詳細を見る

• The β-1,4-mannanase 5C gene (man5C) of Vibrio sp. strain MA-138 was cloned and expressed in Escherichia coli. The man5C gene consisted of 2,010 bp nucleotides encoding a protein of 669 amino acids with a predicted molecular weight of 76,309. β-1,4-Mannanase (Man5C) is a modular enzyme composed of a catalytic module belonging to glycoside hydrolase family 5, a linker region, and a putative carbohydrate-binding module (CBM) belonging to family 27. Recombinant Man5C exhibited maximal activity at 50 °C at pH 7.0, and it had a Km of 0.6 mg ml−1 and a Vmax of 556.2 μmol min−1 μmol−1 for glucomannan. Binding studies revealed that the C-terminal putative CBM27 had the ability to bind soluble β-mannans and contributed to increasing the rate of depolymerization by binding to the polymeric substrate. Man5C of Vibrio sp. MA-138 is the first non-extremophile enzyme to be identified as a β-mannanase possessing CBM27.

• 社団法人 日本農芸化学会の論文
• 2009-01-23

著者

• Tamaru Yutaka

  Graduate School Of Bioresources Mie Univ.

• Araki Toshiyoshi

  Graduate School of Bioresources, Mie University

• Araki Toshiyoshi

  Graduate School Of Bioresources Mie University

• Tanaka Megumi

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Tanaka Megumi

  Graduate School Of Bioresources Mie University

• UMEMOTO Yoshiaki

  Graduate School of Bioresources, Mie University

• OKAMURA Hidenori

  Graduate School of Bioresources, Mie University

• NAKANO Daiichirou

  Graduate School of Bioresources, Mie University

• Araki Toshiyoshi

  Graduate School Of Bioresources Mie Univ.

• Tamaru Yutaka

  Graduate School Of Bioresources Mie University

• Umemoto Yoshiaki

  Graduate School Of Bioresources Mie University

• Okamura Hidenori

  Graduate School Of Bioresources Mie University

• Nakano Daiichirou

  Graduate School Of Bioresources Mie University

関連論文

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• Cloning and Characterization of a β-1, 4-Mannanase 5C Possessing a Family 27 Carbohydrate-Binding Module from a Marine Bacterium, Vibrio sp. Strain MA-138
• Molecular Cloning, Expression, and Characterization of a β-Agarase Gene, agaD, from a Marine Bacterium, Vibrio sp. Strain PO-303

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