Genotyping of a Miso and Soy Sauce Fermentation Yeast, Zygosaccharomyces rouxii, Based on Sequence Analysis of the Partial 26S Ribosomal RNA Gene and Two Internal Transcribed Spacers

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概要

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• We analyzed sequences of the D1D2 domain of the 26S ribosomal RNA gene (26S rDNA sequence), the internal transcribed spacer 1, the 5.8S ribosomal RNA gene, and the internal transcribed spacer 2 (the ITS sequence) from 46 strains of miso and soy sauce fermentation yeast, Zygosaccharomyces rouxii and a closely related species, Z. mellis, for typing. Based on the 26S rDNA sequence analysis, the Z. rouxii strains were of two types, and the extent of sequence divergence between them was 2.6%. Based on the ITS sequence analysis, they were divided into seven types (I–VII). Between the type strain (type I) and type VI, in particular, a 12% difference was detected. The occurrence of these nine genotypes with a divergence of more than 1% in these two sequences suggests that Z. rouxii is a species complex including novel species and hybrids. Z. mellis strains were of two types (type α and type β) based on the ITS sequence. Z. rouxii could clearly be distinguished from Z. mellis by 26S rDNA and ITS sequence analyses, but not by the 16% NaCl tolerance, when used as the sole key characteristic for differentiation between the two species.

• 社団法人 日本農芸化学会の論文
• 2008-09-23

著者

• Suezawa Yasuhiko

  Fermentation And Food Res. Branch Kagawa Prefectural Industrial Center

• Suezawa Yasuhiko

  Fermentation And Food Research Branch Kagawa Prefectural Industrial Center

• Suzuki Motofumi

  Microbe Division/japan Collection Of Microorganisms Bioresource Center Riken

• MORI Haruhiko

  Siegfried Laboratory

関連論文

• Identification and Typing of Miso and Soy Sauce Fermentation Yeasts, Candida etchellsii and C. versatilis, Based on Sequence Analyses of the D1D2 Domain of the 26S Ribosomal RNA Gene, and the Region of Internal Transcribed Spacer 1, 5.8S Ribosomal RNA Gen
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• Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces
• Genotyping of a Miso and Soy Sauce Fermentation Yeast, Zygosaccharomyces rouxii, Based on Sequence Analysis of the Partial 26S Ribosomal RNA Gene and Two Internal Transcribed Spacers

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