Isolation and Identification of a Thermophilic Strain Producing Trehalose Synthase from Geothermal Water in China
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概要
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A slightly thermophilic strain, CBS-01, producing trehalose synthase (TreS), was isolated from geothermal water in this study. According to the phenotypic characteristics and phylogenetic analysis of the 16s rRNA gene sequence, it was identified as Meiothermus ruber. The trehalose synthase gene of Meiothermus ruber CBS-01 was cloned by polymerase chain reaction and sequenced. The TreS gene consisted of 2,895 nucleotides, which specified a 964-amino-acid protein. This novel TreS catalyzed reversible interconversion of maltose and trehalose.
- 社団法人 日本農芸化学会の論文
- 2008-08-23
著者
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Zhang Jun
Tianjin Horticultural Engineering Institute
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Xing Laijun
Key Laboratory Of Molecular Microbiology And Technology Ministry Of Education Department Of Microbio
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ZHU Yueming
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microb
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WEI Dongsheng
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microb
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WANG Yufan
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microb
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CHEN Xiaoyun
Tianjin Horticultural Engineering Institute
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LI Mingchun
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microb
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Wang Yufan
Key Laboratory Of Molecular Microbiology And Technology Ministry Of Education Department Of Microbio
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Wei Dongsheng
Key Laboratory Of Molecular Microbiology And Technology Ministry Of Education Department Of Microbio
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Li Mingchun
Key Laboratory Of Molecular Microbiology And Technology Ministry Of Education Department Of Microbio
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Zhu Yueming
Key Laboratory Of Molecular Microbiology And Technology Ministry Of Education Department Of Microbio