Expression and Characterization of the Streptomyces coelicolor Serine/Threonine Protein Kinase PkaD
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概要
- 論文の詳細を見る
We identified and characterized the gene encoding a new eukaryotic-type protein kinase from Streptomyces coelicolor A3(2) M145. PkaD, consisting of 598 amino acid residues, contained the catalytic domain of eukaryotic protein kinases in the N-terminal region. A hydrophobicity plot indicated the presence of a putative transmembrane spanning sequence downstream of the catalytic domain, suggesting that PkaD is a transmembrane protein kinase. The recombinant PkaD was found to be phosphorylated at the threonine and tyrosine residues. In S. coelicolor A3(2), pkaD was transcribed as a monocistronic mRNA, and it was expressed constitutively throughout the life cycle. Disruption of chromosomal pkaD resulted in a significant loss of actinorhodin production. This result implies the involvement of pkaD in the regulation of secondary metabolism.
- 社団法人 日本農芸化学会の論文
- 2008-03-23
著者
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Ogawara Hiroshi
Department Of Biochemistry Meiji College Of Pharmacy
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Ogawara Hiroshi
Department Of Biochemistry Meiji Pharmaceutical University
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Urabe Hiroaki
Department Of Biochemistry Meiji Pharmaceutical University
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Urabe Hiroaki
Department Of Biochemistry Meiji College Of Pharmacy
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Motojima Kiyoto
Department Of Biochemistry Meiji Pharmaceutical University
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AOYAGI Narumi
Department of Biochemistry, Meiji Pharmaceutical University
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Aoyagi Narumi
Department Of Biochemistry Meiji Pharmaceutical University
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Ogawara Hiroshi
Department Of Biochemistry Ii Meiji College Of Pharmacy
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Motojima Kiyoto
Department of Biochemistry, Meiji Pharmaceutical University
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