Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011
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概要
- 論文の詳細を見る
Methane monooxygenase hydroxylase was purified by chromatography and characterized by electrophoresis and spectroscopy. The molecular mass of hydroxylase was 201.3 KDa as determined by gel filtration, whereas the total molecular mass was 234 KDa as judged by SDS–PAGE. Structure study indicated that the enzyme is a homodimer structure, consisting of three subunits, designated α, β, and γ, with molecular masses of 58 KDa, 36 KDa, and 23 KDa respectively. IEF analysis indicated that the enzyme has a pI of 5.2. The UV–Vis spectrum of hydroxylase revealed an absorption peak near 281 nm and a weak shoulder peak around 395 nm–420 nm, and a fluorescence spectrum revealed an emission peak at 341.3 nm. Circular dichroism measurement indicated that hydroxylase mainly consists of α-helical regions. Finally, phylogenetic analysis indicated that this strain is very close to Methylosinus trichosporium OB3b.
- 社団法人 日本農芸化学会の論文
- 2007-01-23
著者
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Li Shuben
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Niu Jianzhong
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Tang Wei
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Li Shuben
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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HUA SHAOFENG
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physic
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XIN JIAYIN
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physic
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XIA CHUNGU
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physic
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TAN HAIDONG
Dalian Institute of Chemical Physics, Chinese Academy of Sciences
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Xin Jiayin
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Xia Chungu
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Tan Haidong
Dalian Institute Of Chemical Physics Chinese Academy Of Sciences
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Hua Shaofeng
State Key Laboratory For Oxo Synthesis And Selective Oxidation Lanzhou Institute Of Chemical Physics
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Shaofeng Hua
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences
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Jiayin Xin
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences
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Shuben Li
State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences
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- Purification and Biochemical Characterization of Soluble Methane Monooxygenase Hydroxylase from Methylosinus trichosporium IMV 3011