Acid-Sensing Ion Channel 3, But Not Capsaicin Receptor TRPV1, Plays a Protective Role in Isoproterenol-Induced Myocardial Ischemia in Mice

スポンサーリンク

概要

• 論文の詳細を見る

• Background: Cardiac angina is the hallmark of myocardial ischemia, but the role of the cardiac sensory nerve has received relatively little attention. Recently, both acid-sensing ion channel 3 (ASIC3) and capsaicin receptor (TRPV1) have been suggested as important mediators in sensing cardiac ischemia. However, studies comparing the physiological roles of ASIC3 and TRPV1 in the neuronal-cardiac sensing circuits in vivo are lacking. Methods and Results: Isoproterenol (1.5mg/kg, intraperitoneally) was used to induce transient cardiac ischemia in Asic3+/+ and Asic3-/- mice and a radio-telemetry system was used for electrocardiography with mice in a conscious state. Isoproterenol-induced cardiac ischemia was first demonstrated with ST-segment depression and further confirmed by hypoxia-mediated chemical reactions in cardiac tissue. Mice lacking Asic3 showed prolonged duration of ST-segment depression compared with Asic3+/+ mice (44.3±3.1 vs. 31.7±2.9min; P<0.05). Although ischemia was transient, severe cardiac fibrosis was found in Asic3-/- but not in Asic3+/+ mice littermates. In contrast, isoproterenol-injected Trpv1+/+ and Trpv1-/- mice showed no difference in duration of ST-segment depression and, surprisingly, deletion of Trpv1 did not aggravate cardiac fibrosis. Conclusions: An isoproterenol-induced cardiac ischemia model mimicking clinical conditions of early cardiac angina was used to demonstrate that ASIC3 but not TRPV1 plays a protective role in sensing cardiac ischemia. (Circ J 2011; 75: 174-178)

• 2010-12-25

著者

• Cheng Ching-feng

  Institute Of Biomedical Sciences Academia Sinica

• Kuo Terry

  Institute of Brain Science, Yang Ming University

• Chen Chih-Cheng

  Institute of Biomedical Sciences, Academia Sinica

• Kuo Terry

  Institute Of Brain Sciences Yang Ming University

• Cheng Mei-hsin

  Institute Of Biomedical Sciences Academia Sinica

• Chen I-lun

  Institute Of Biomedical Sciences Academia Sinica

• Lian Wei-shiung

  Institute Of Biomedical Sciences Academia Sinica

• Lin Chao-chieh

  Institute Of Biomedical Sciences Academia Sinica

• Chen Chih-cheng

  Institute Of Biomedical Sciences Academia Sinica

• Kuo Terry

  Institute Of Brain Science Yang Ming University

関連論文

• PE-036 Abnormal cardiac autonomic regulation and susceptibility to cardiac ischemia in mice lack of ASIC3(Autonomic nervous system(03)(H),Poster Session(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• Acid-Sensing Ion Channel 3, But Not Capsaicin Receptor TRPV1, Plays a Protective Role in Isoproterenol-Induced Myocardial Ischemia in Mice
• Role of the Acid-Sensing Ion Channel 3 in Blood Volume Control
• Thromboxane A2 Mediates Iron-Overload Cardiomyopathy in Mice Through Calcineurin-Nuclear Factor of Activated T Cells Signaling Pathway

スポンサーリンク