蛋白質不含培地におけるブタ卵母細胞の成熟におよぼす17β-estradiolの影響

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概要

• 論文の詳細を見る

• The present study examined the effects of 17 β-estradiol (E2) on in vitro maturation and subsequent in vitro fertilization of pig oocytes matured with or without cumulus cells. When E2 (10 ng/ml) was added to the protein-free maturation medium, the proportions of cumulus-enclosed oocytes that underwent germinal vesicle breakdown and reached metaphase II were significantly reduced (P<0.05), and cumulus expansion was also significantly inhibited (P<0.05) compared with the control (no E2 added). Although oocytes matured in the presence of E2 were penetrated by sperm in vitro at the same level as the control, the incidences of male pronuclear (MPN) formation and activated oocytes were significantly lower (P<0.05) than the control. These inhibitory effects of E2 were prevented when the medium was supplemented with E2 together with its antagonist, ICI 182,780 (1 μg/ml), although the presence of the antagonist alone in the medium had no effect on the maturation and fertilization in vitro of oocytes. In cumulus-free oocytes, E2 had no effect on nuclear maturation and penetration in vitro, but low MPN formation was observed in oocytes matured in the presence and absence of E2. When cumulus-enclosed oocytes were cultured in the presence of progesterone (P4; 600 ng/ml) alone or together with E2, no significant differences in nuclear maturation, cumulus expansion or penetration in vitro were observed compared with control oocytes. The concentration of P4 in maturation medium was significantly (P<0.01) lower when cumulus-enclosed oocytes were cultured for 44 h in the medium with E2 than in medium without E2. These results indicate that E2 inhibits both nuclear and cytoplasmic maturation of cumulus-enclosed pig oocytes, and that this inhibition can be prevented by an E2 antagonist or P4. This E2 inhibition may occur indirectly via the cumulus cells and inhibition of P4 synthesis.

• 日本繁殖生物学会の論文
• 2004-06-01

著者

• NIWA Koji

  The Graduate School of Natural Science and Technology

• Niwa Koji

  The Graduate School Of Natural Science And Technology Okayama University

• LI Qing

  The Graduate School of Natural Science and Technology, Okayama University

• Hunter Morag

  Division Of Animal Physiology School Of Biosciences University Of Nottingham

• Li Qing

  The Graduate School Of Natural Science And Technology Okayama University

• 丹羽 晧二

  The Graduate School of Natural Science and Technology, Okayama University

• 李 青

  The Graduate School of Natural Science and Technology, Okayama University

関連論文

• ラット胚の体外発生におよぼす牛血清アルブミン(BSA)および牛胎仔血清(FBS)の影響
• 体外成熟豚卵子透明帯へのレクチンの結合と体外における精子の接着
• 卵胞膜細胞との共培養による卵丘付着牛卵母細胞の卵核胞発育阻害を伴わない減数分裂の休止
• カルシウム・イオノフォア(CaA)で処理された豚卵子の活性化と体外受精
• 蛋白質不含培地におけるブタ卵母細胞の成熟におよぼす17β-estradiolの影響
• 卵胞膜細胞との共培養による卵丘付着牛卵母細胞の卵核胞発育阻害を伴わない減数分裂の休止
• 透明帯との相互作用に関与する凍結-融解豚射出精子におけるグリコシダーゼの同定と局在性
• 蛋白質不含培地におけるブタ卵母細胞の成熟におよぼす17β-estradiolの影響

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