Effect of Bacterial Media on the Evaluation of the Antibacterial Activity of a Biomaterial Containing Inorganic Antibacterial Reagents or Antibiotics

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概要

• 論文の詳細を見る

• Several studies have been performed to assess the effectivesness of the antibacterial coating of a biomaterial to reduce surgical site infection. However, evaluations of these materials are inconsistent, and therefore it is difficult to compare their antibacterial performance. In this study, we evaluated the influence of different media such as nutrient broth (NB), Mueller-Hinton broth (MHB) and fetal bovine serum (FBS) on the antibacterial activity of AgNO3- or gentamicin-added bone cement using a modified ISO 22196 standard to devise a method to evaluate the antibacterial activity of biomaterials in vitro. The antibacterial activity results against Staphylococcus aureus and Escherichia coli were different in each medium. The antibacterial activity of AgNO3 in FBS was lower than the other media, whereas the antibacterial activity of gentamicin in FBS was higher than in the other media. It was assumed that the fluctuating antibacterial activity was influenced by serum components. The results showed that the ISO 22196 antibacterial evaluation method is suitable to evaluate antibacterial biomaterials after modifying the medium to FBS.

• 日本防菌防黴学会の論文
• 2010-03-10

著者

• HOTOKEBUCHI TAKAO

  Department of Orthopaedic Surgery, Faculty of Medicine, Kyushu University

• Hotokebuchi Takao

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

• Ando Yoshiki

  Division Of Microbiology Department Of Pathology And Microbiology Faculty Of Medicine Saga Universit

• MAWATARI MASAAKI

  Department of Orthopaedic Surgery, Faculty of Medicine, Saga University

• MIYAMOTO Hiroshi

  Division of Biodefense, Departments of Pathology and Biodefense, Faculty of Medicine, Saga Universit

• MIYAZAKI Masaki

  Department of Immunology, Graduate School of Biomedical Sciences, Hiroshima University

• NODA IWAO

  Research Department, Japan Medical Materials Corporation

• MIYAJI FUMIAKI

  Research Department, Japan Medical Materials Corporation

• SHIMAZAKI TAKAFUMI

  Department of Orthopaedic Surgery, Faculty of Medicine, Saga University

• YONEKURA YUTAKA

  Department of Orthopaedic Surgery, Faculty of Medicine, Saga University

• Hotokebuchi Takao

  Department Of Advanced Medicine National Center For Geriatrics And Gerontology

• Miyazaki Masaki

  Department Of Immunology Graduate School Of Biomedical Sciences Hiroshima University

• Miyazaki Masaki

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

• Miyaji Fumiaki

  Research Department Japan Medical Materials Corporation

• Mawatari Masaaki

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

• Noda Iwao

  Research Department Japan Medical Materials Corporation

• Miyamoto Hiroshi

  Division Of Microbiology Department Of Pathology And Microbiology Faculty Of Medicine Saga Universit

• Yonekura Yutaka

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

• Shimazaki Takafumi

  Department Of Orthopaedic Surgery Faculty Of Medicine Saga University

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