Antiplasmodial effects of Brucea javanica (L.) Merr. and Eurycoma longifolia Jack extracts and their combination with chloroquine and quinine on Plasmodium falciparum in culture

スポンサーリンク

概要

• 論文の詳細を見る

• Fruits of Brucea javanica (L.) Merr. (“Ratchadad” in Thai) and roots of Eurycoma longifolia Jack (“Plalaipeag” in Thai) are used as traditional medicines for the treatment of malarial fever. Ethanol, methanol, ethyl acetate, ethyl alcohol and aqueous extracts were tested against the multidrug-resistant Plasmodium falciparum strain K1. Ethanol and methanol-ethanol extracts, together with methanol residue, from fruits of B. javanica (L.) Merr. showed the highest antiplasmodial activities with IC50 values of 0.5 ± 0.3, 0.3 ± 0.1 and 0.3 ± 0.05 Μg⁄mL, respectively, comparable to the IC50 values of chloroquine (0.17 ± 0.02 Μg⁄mL) and quinine (0.3 ± 0.1 Μg⁄mL). Similarly, ethanol and methanol-ethanol extracts of roots of E. longifolia Jack showed higher activities than those of the other solvent extracts, but their activities were about 10-fold lower than those of extracts from B. javanica (L.) Merr. fruit. In drug combination tests, B. javanica (L.) Merr. and E. longifolia Jack extracts did not appear to antagonize antiplasmodial activity of chloroquine and quinine. Not only well-known quassinoid glycosides but also coumarins and flavonoids identified by thin-layer chromatography in ethanol and methanol-ethanol extracts and in methanol residue of B. javanica (L.) Merr fruit and E. longifolia roots may be responsible for the antimalarial activity. Taken together, our extraction conditions provided extracts containing novel active compounds that did not antagonize the inhibitory effects of the two widely used antimalarials. This finding could lend support to the future discovery of active antimalaria compounds of Brucea javanica (L.) Merr. and Eurycoma longifolia Jack as drugs for the treatment of malaria that could be employed as drug combinations in order to delay the onset of parasite drug resistance.

• 2010-06-01

著者

• Sriwilaijaroen Nongluk

  Faculty Of Medicine Thammasat University

• Wilairat Prapon

  Department Of Biochemistry Faculty Of Science Mahidol University

• KONDO Sumalee

  Faculty of Medicine, Thammasat University

• NANTHASRI Pranee

  Faculty of Medicine, Thammasat University

• AUPARAKKITANON Saranya

  Division of Toxicology, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol

• SUZUKI Yasuo

  Health Science Hills, College of Life and Health Sciences, Chubu University

• Kondo Sumalee

  Faculty Of Medicine Thammasat University

• Nanthasri Pranee

  Faculty Of Medicine Thammasat University

• Auparakkitanon Saranya

  Division Of Toxicology Department Of Pathology Faculty Of Medicine Ramathibodi Hospital Mahidol Univ

• Suzuki Yasuo

  Health Science Hills College Of Life And Health Sciences Chubu University

関連論文

• Antiplasmodial effects of Brucea javanica (L.) Merr. and Eurycoma longifolia Jack extracts and their combination with chloroquine and quinine on Plasmodium falciparum in culture
• Partial purification and characterization of mitochondrial DNA polymerase from Plasmodium falciparum
• Gametocytosidal activity of pyronaridine and DNA topoisomerase II inhibitors against multidrug-resistant Plasmodium falciparum in vitro
• Hemoglobin Pakse : Presence on Red Blood Cell Membrane and Detection by Polymerase Chain Reaction-Single-Strand Conformational Polymorphism
• Molecular basis of the structure and function of H1 hemagglutinin of influenza virus
• Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells

スポンサーリンク