DNAやペプチドの自己集合によるナノバイオ分子システムの構築

概要

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In biological system, various nano–assemblies are formed by self–assembly of proteins with high symmetric ternary structures. For example, dodecahedral internal skeleton of tomato bushy stunt virus is self–assembled from C3–symmetric β–sheet subunits. Inspired by such self–assembly process, we have developed a novel strategy for construction of spherical assemblies consisting of biomacromolecules such as DNA and peptides. DNA three–way junctions bearing self–complementary sticky–ends were self–assembled into nanometer to micrometer sized spherical structures depending on the concentration. The three–way component design was extended to the design of the artificial C3–symmetric conjugate which contains three β–sheet–forming peptides FKFEFKFE. Radial–type Trigonal–FKFE self–assembled into nanocapsules with the size of 20 nm. In contrast, wheel–type Trigonal–FKFE self–assembled into nanofibers with uniform width (3 nm). Recently, we designed T–shaped C3–symmetric peptide conjugate Trigonal–glutathiones (TG). TG self–assembled into soft nanospheres with the size of about 100—250 nm, whose size was little affected by the concentration.
2010-04-01