5-ブロモ, 5-ヨードウラシルを含むDNAの光反応 : DNAの構造と電子移動の解析への応用

概要

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Photoreactivities of 5–bromouracil– or 5–iodouracil–containing DNA have widely been used as DNA–protein photocrosslinking to investigate their interface. We have accumulated the photoreactivities of 5–bromouracil or 5–iodouracil in various DNA local structures. It was found that hydrogen abstraction by uracil–5–yl radical is highly conformation–dependent to provide unique oxidative damage sites. The results suggest that this photochemical method can be used to detect DNA local structures. Recently, 5–bromouracil– or 5–iodouracil–containing DNA has also been used as probe of DNA charge transfer, since one–electron reduction is necessary for the formation of uracil–5–yl from 5–bromouracil–containing DNA. In this review, we discuss specific hydrogen abstraction by uracil–5–yl radical in various DNA local structures and electron transfer processes along DNA strand in B– and Z–form as well as between DNA–protein interface.
社団法人有機合成化学協会の論文
2009-12-01