Three-Dimensional Super-Resolution Microscope Using Two-Color Annular Phase Plate

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概要

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• To achieve three-dimensional super-resolution microscopy (3D-SRM) using fluorescence depletion, a two-color annular phase plate has been applied to a commercial laser-scanning microscope (LSM). When two-color beams passing through the plate are focused, only one of the beams is modulated, so that a minute 3D-dark-hole is generated at the focal point. Using these two beams for illumination lights for 3D-SRM, both the lateral and the axial resolutions overcame the diffraction limit. Inserting the plate into the optical pass for illumination in an LSM, the setup of 3D-SRM works as a very simple and compact analysis tool in life and material science.

• 2010-08-25

著者

• IKETAKI Yoshinori

  PRESTO "Light and Material" Japan Science and Technology Agency

関連論文

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• Three-Dimensional Super-Resolution Microscope Using Two-Color Annular Phase Plate

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