Enhanced solubilization of polygalacturonic acid synthase by ribonuclease treatment of cell homogenates

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概要

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• The glycosyltransferases involved in plant cell wall biosynthesis are generally too low in concentration to allow for their purification. Therefore, the development of a method for enhanced solubilization of membrane proteins is necessary. Here, we report ribonuclease treatment of cell homogenates of azuki bean epicotyls. Polygalacturonic acid synthase, localized at the plant Golgi membrane, was enriched 2.0-fold in specific activity after ribonuclease treatment. Total enzyme activity per unit weight was also improved 2.1-fold. These data indicate that ribonuclease works to remove ribosomes from the microsomal fraction and enhances the solubilization of polygalacturonic acid synthase.

• 2010-09-06

著者

• Ishimizu Takeshi

  Graduate School Of Science Osaka University

• Yasui Kazutoshi

  Graduate School Of Science Osaka University

• UEGAKI Masamichi

  Graduate School of Science, Osaka University

• Uegaki Masamichi

  Graduate School Of Science Osaka University

関連論文

• Enhanced solubilization of polygalacturonic acid synthase by ribonuclease treatment of cell homogenates
• Molecular evidence that most RNAs required for germination and pollen tube growth are stored in the mature pollen grain in petunia
• UDP-Glucose Pyrophosphorylase is Rate Limiting in Vegetative and Reproductive Phases in Arabidopsis thaliana
• Molecular evidence that most RNAs required for germination and pollen tube growth are stored in the mature pollen grain in petunia

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