Ultrasonographic Fetal Sex Identification in Pregnant Sheep Derived from Natural Mating and Embryo Transfer
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概要
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The objective of this study was to identify the migration period of the genital tubercle and the period of visualization of external genital structures in fetuses of the Dorper breed of sheep derived from natural mating and from fresh, frozen and vitrified embryo transfer. Transrectal ultrasound was performed using a double-frequency linear transducer (6.0 and 8.0 MHz) to monitor 130 ewe fetuses distributed in the four treatments regarding embryo origin. The accuracy of the ultrasound was 100% in this experiment. The fetuses originated from controlled natural mating (NM) and from fresh (FrE), frozen (FE) and vitrified (VE) embryo transfer, with embryos collected 7 days after breeding. Migration of the genital tubercle occurred earlier (P<0.05) in NM (42.21 ± 2.86 days) than in FrE (43.98 ± 3.00 days), FE (44.97 ± 1.83 days) and VE (44.58 ± 1.97 days). Visualization of the scrotal bag, prepuce and vulva occurred, respectively, earlier (P<0.05) in NM (45.22 ± 1.25, 45.95 ± 1.53 and 45.01 ± 1.03 days) than in FrE (48.91 ± 1.92, 48.52 ± 1.41 and 47.41 ± 1.41 days), FE (49.97 ± 1.08, 49.18 ± 2.00 and 47.64 ± 1.82 days) and VE (50.12 ± 1.66, 49.27 ± 1.61 and 47.93 ± 1.92 days). The results show that fetal sexing can be accomplished from the 50th day onward in fetuses produced by natural mating and from the 55th day onward in fetuses derived from fresh, frozen and vitrified embryos. It can also be concluded that real-time ultrasonography is a reliable tool for fetal sex determination in sheep taking into account both the location of the genital tubercle and the identification of external genital structures.
- 2010-06-01
著者
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DE OLIVEIRA
Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao
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Freitas Neto
Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Univers
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Santos Maico
Bolsita (BFP) da Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
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Aguiar Filho
Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Univers
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CALDAS Eduardo
Diagnóstico por Imagem do Hospital Veterinário da Faculdade Pio Décimo
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NEVES Jairo
Diagnóstico por Imagem do Hospital Veterinário da Faculdade Pio Décimo
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LIMA Paulo
Laboratório de Biotécnicas Aplicadas à Reprodução do Departamento de Medicina Veterinária da Univers
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De Freitas
Laboratorio De Biotecnicas Aplicadas A Reproducao Do Departamento De Medicina Veterinaria Da Univers
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De Almeida
Laboratorio De Biotecnicas Aplicadas A Reproducao Do Departamento De Medicina Veterinaria Da Univers
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De Oliveira
Laboratorio De Biotecnicas Aplicadas A Reproducao Do Departamento De Medicina Veterinaria Da Univers
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De Aguiar
Laboratorio De Primatologia Faculdade De Biociencias Pontificia Universidade Catolica Do Rio Grande
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Dos Santos
Bolsita (bfp) Da Fundacao De Amparo A Ciencia E Tecnologia Do Estado De Pernambuco (facepe)
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De Aguiar
Laboratorio De Biotecnicas Aplicadas A Reproducao Do Departamento De Medicina Veterinaria Da Univers
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De Almeida
Laboratorio De Biologia Celular Nucleo De Pesquisa Multidisciplinar Instituto De Ciencias Biologicas
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De Oliveira
Laboratorio De Biologia Celular E Tecidual Centro De Biociencias E Biotecnologia Universidade Estadu
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