紅斑性および偽膜性カンジダ性舌炎から分離された Candida albicans に対するヒト歯肉上皮細胞の細胞応答

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Among patients receiving treatment in dental clinics, those complaining of chronic superficial glossodynia are frequently encountered in clinical practice. It was recently revealed that most of these painful episodes are caused by candida infection, and as a treatment strategy, episodes of glossodynia can be relatively easily relieved by antifungal mouthwash or its oral preparation. Candidal glossodynia can be classified into two groups based on clinical findings, that is, glossodynia accompanied by erythema due to atrophied lingual membrane; erythematous candidiasis and that accompanied by pseudomembrane adhered to the membrane surface; pseudomembranous candidiasis.In this study, the potential difference between 5 strains isolated from 5 erythematous candidiasis and 5 strains isolated from 5 pseudomembranous candidiasis among patients at random. The drug sensitivity test previously performed by using four kinds of antifungal drug, miconazole, itraconazole, amphotericin B and nystatin, all of the drugs showed good drug sensitivity against the 10 strains of C. albicans isolated, besides of the subsequent test on the biochemical properties using a fungal identification kit for pathogenic yeasts, Api-C-Auxanogram®, no significant difference was detected between the two groups examined.Then it was examined by referring to the various cellular responses to human gingival epithelial cells derived from isolated Candida albicans.Individual C. albicans strains isolated in the two groups; 5 strains each, were then sensitized with cultured keratinocytes in order to determine the levels of inflammatory cytokines including Interleukin 1β, Interleukin 6, and Interleukin 8 by ELISA method, for comparison.In the present study, however, there was no difference in the time to expression of IL-6 and IL-8 between the erythromatous and pseudomembranous groups and both indexes were found to have increased significantly during the period of sensitization from 3 to 12 hours. In addition, the production of cytokines was promoted in the pseudomembranous candidiasis group as compared with the erythromatous candidiasis group with a significant difference noted between the two groups.From the evidence mentioned above, there is a distinct difference in the cytokine levels produced by the isolated fungi by referring to the clinical symptoms in relation to the inflammatory statuses between erythromatous and pseudomembranous candidal glossodynia, besides the various clinical findings detected by visual examination.
日本歯科薬物療法学会の論文
2009-08-01

紅斑性および偽膜性カンジダ性舌炎から分離された Candida albicans に対するヒト歯肉上皮細胞の細胞応答

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