Relationship between Caspase Activity and Apoptotic Markers in Human Sperm in Response to Hydrogen Peroxide and Progesterone
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概要
- 論文の詳細を見る
Apoptosis plays an essential role in normal spermatogenesis, but deregulations of this biological process, which is closely associated with male infertility, have been found. Whereas calcium homeostasis is a key regulator of cell survival, sustained elevation of intracellular calcium plays a role in apoptosis. The aim of this research was to determine the role of two different calcium mobilizing agents, hydrogen peroxide (H2O2) and the physiological agonist progesterone, on the apoptosis process of human ejaculated spermatozoa. Translocation of membrane phosphatidylserine was examined with an annexin V binding assay, DNA damage was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL assay) and caspase-3 activity was assessed using a fluorometric assay. After incubation of spermatozoa for 1 h with either 10 μM H2O2 or 20 μM of progesterone, there was a significant increase in both caspase-3 activity and the percentage of annexin V-positive cells. Similarly, the TUNEL results were significantly higher 1 h after incubation with either 10 μM H2O2 or 20 μM of progesterone. In fact, progesterone-treated cells showed a three-fold increase (from 17.6 to 52.9%) of TUNEL-positive cells compared to untreated cells, while H2O2-treated cells exhibited a two-fold increase (from 17.6 to 37.9%). In sum, our results suggest that spermatozoa treated with calcium mobilizing agents, such as H2O2 and progesterone, seem to undergo an apoptosis process that is dependent on caspase-3 activation.
- 日本繁殖生物学会の論文
- 2009-12-01
著者
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Ortiz Agueda
Extremadura Center Of Human Assisted Reproduction Infantile Hospital
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Espino Javier
Department Of Physiology University Of Extremadura
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Rodriguez Ana
Extremadura Center Of Human Assisted Reproduction Infantile Hospital
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Lozano Graciela
Extremadura Center Of Human Assisted Reproduction Infantile Hospital
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BEJARANO Ignacio
Department of Physiology, University of Extremadura
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GONZALEZ David
Department of Physiology, University of Extremadura
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GARCIA Juan
Department of Physiology, University of Extremadura
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PARIENTE Jose
Extremadura Center of Human Assisted Reproduction, Infantile Hospital
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Pariente Jose
Extremadura Center Of Human Assisted Reproduction Infantile Hospital
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Bejarano Ignacio
Department Of Physiology University Of Extremadura
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Gonzalez David
Department Of Physiology University Of Extremadura
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Garcia Juan
Department Of Physiology University Of Extremadura
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Gonzalez David
Department Of Haemato-oncology Royal Marsden Hospital
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Gonzalez David
Departamento De Ciencia De Los Materiales E I. M. Y Q. I. Universidad De Cadiz
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- Relationship between Caspase Activity and Apoptotic Markers in Human Sperm in Response to Hydrogen Peroxide and Progesterone
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