Quantitation of Fungal DNA Contamination in Commercial Zymolyase and Lyticase Used in the Preparation of Fungi
スポンサーリンク
概要
- 論文の詳細を見る
Small amounts of contaminants may lead to false-positive results in sensitive polymerase chain reaction (PCR) detection systems. To analyze contaminants and understand the usability of β-glucanases in fungal preparations, we estimated the ribosomal DNA (rDNA) contamination in Zymolyase-100T and Lyticase by quantitative PCR. The amount of rDNA contamination determined by real-time PCR was 9210 copies/unit for Zymolyase-100T and 0.0323 copies/unit for Lyticase. The observations regarding these enzyme products indicate that careful consideration of contaminating DNA included in the reagents used for molecular diagnostics is necessary.
- 日本医真菌学会の論文
- 2009-10-30
著者
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Miyajima Yoshiharu
Teikyo Univ. Inst. Of Medical Mycology
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Miyajima Yoshiharu
Teikyo University Institute Of Medical Mycology
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Makimura Koichi
Teikyo University, Institute of Medical Mycology
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Satoh Kazuo
帝京大学 医学部内科学講座
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SATOH Kazuo
Teikyo University Institute of Medical Mycology
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UMEDA Yoshiko
Teikyo University Institute of Medical Mycology
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Makimura Koichi
Teikyo Univ. Inst. Of Medical Mycology
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