Cloning and tissue-specific expression of a δ-COP homologue in a freshwater and a brackish water-adapted strain of rainbow trout : Oncorhynchus mykiss

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In eukaryotic cells, intracellular transport is mediated by coated vesicular carriers. Coat proteins I (COPI) vesicles are involved in the retrograde transport from Golgi apparatus to the endoplasmic reticulum. The COPI complex is composed of ADP-ribosylation factor 1 and coatomer comprising seven subunits, termed α–ζ. We isolated and characterised a cDNA sequence from rainbow trout homolog to δ-subunit of COPI complex (δ-COP). Trout δ-COP gene encodes a protein of 509 aa including a characteristic Mu homology domain. Searches at the Ensemble Genome browser identified three additional teleostean δ-COP-like sequences from pufferfish, rice fish, and stickleback. Sequence identity of piscine δ-COP protein sequences is greater than 84%. Moreover, a phylogenetic analysis indicates that δ-COP protein sequences are strongly conserved among vertebrate species. δ-COP homologue is ubiquitously expressed in trout tissues. Quantitative Real-Time RT-PCR revealed that δ-COP is differentially expressed in liver and gill tissue of two rainbow trout strains, the freshwater strain STEELHEAD and the brackish water-adapted strain BORN.
2009-06-25