形態と細胞機能の研究手法 : 単離細胞実験法

概要

論文の詳細を見る
Studies of the inner ear have been extended to the level of the single cells, and physiological studies, in particular, have advanced to the use of isolated inner ear living cells: i.e., outer hair cells (OHCs), inner hair cells (IHCs), Deiters cells, and Hensens cells. Here, we describe a rapid isolation technique for inner ear cells from the guinea-pig cochlea. Some proteolytic enzymes used for the isolation were found to affect the properties of the K currents in the isolated IHCs. While extracellular application of sodium salicylate induced an acute change in the shape of the isolated OHCs. Dexamethasone suppressed the salicylate-induced morphological changes of the cells. We describe the device used to perfuse the patch electrode for exchanging the intracellular medium of the isolated cells.
日本めまい平衡医学会の論文
2008-06-01