Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy

スポンサーリンク

概要

• 論文の詳細を見る

• Background: Mutations of cardiac troponin C (cTnC) can cause dilated cardiomyopathy in humans. Methods and Results: Plasmids were constructed such that the reverse tetracycline-controlled transactivator (rtTA) was driven by the cardiac myosin light chain 2 promoter. This heart-specific rtTA bound another bidirectional promoter to express the green fluorescence protein reporter gene and the antisense RNA of cTnC in the presence of doxycycline. A transgenic line of zebrafish (CA17) with cTnC dysfunction was also generated. The heart rates of the embryos in the CA17 line were significantly slower than those of embryos in the control T03 transgenic line at 6 and 12 days post fertilization (dpf). In the CA17 line, cardiac chambers in the F2 embryos were significantly greater and the ventricular ejection fraction was lower than those in the T03 at both 6 and 12 dpf. The mortality rate of F2 adult fish of the CA17 line was also significantly higher (P<0.001). Conclusions: Using conditional expression of antisense RNA of zebrafish cTnC, a new animal model with phenotypes simulating dilated cardiomyopathy has been created.

• 2009-08-20

著者

• Ho Yi-lwun

  Graduate Institute Of Clinical Medicine National Taiwan University Hospital And National Taiwan Univ

• Tsai Wei-yuan

  Institute Of Molecular And Cellular Biology National Taiwan University

• LIN Yen-Hung

  Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and Nat

• HSIEH Fong-Jou

  Department of Obstetrics and Gynecology, National Taiwan University Hospital and National Taiwan Uni

• TSAI Huai-Jen

  Institute of Molecular and Cellular Biology, National Taiwan University

• Lin Yen-hung

  Division Of Cardiology Department Of Internal Medicine National Taiwan University Hospital And Natio

• Hsieh Fong-jou

  Department Of Obstetrics And Gynecology National Taiwan University Hospital And National Taiwan Univ

• Tsai Huai-jen

  Institute Of Molecular And Cellular Biology National Taiwan University

• Tsai Huai-jen

  Institute Of Fisheries Science National Taiwan University

関連論文

• Conditional Antisense-Knockdown of Zebrafish Cardiac Troponin C as a New Animal Model for Dilated Cardiomyopathy
• Transient Expression of Mouse Tyrosinase Gene in Albino Walking Catfish Claria fuscus by Subcutaneous Microinjection
• Functional Analysis of the Proximal Promoter Regions of Fish Rhodopsin and Myf-5 Genes Using Transgenesis
• Effect of Electroporation Conditions on Loach Sperm for Successful Gene Transfer and Early Development
• Vibrio属細菌数種の染色体DNAのフィンガープリント法による識
• 同一分子量で制限酵素切断パターンの異なるVibrio anguillarum のプラスミドDNA

スポンサーリンク