Continuous UV-B Irradiation Induces Endoreduplication and Peroxidase Activity in Epidermal Cells Surrounding Trichomes on Cucumber Cotyledons

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概要

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• Most trichomes on the surface of cucumber (Cucumis sativus L.) cotyledons consist of three cells. We previously showed that continuous UV-B (290 -320 nm) irradiation induces rapid cellular expansion and the accumulation of polyphenolic compounds, possibly stress lignin, in epidermal cells around these trichomes.1) To examine the mechanism of the UV-B-induced cellular expansion and to determine which step is stimulated by UV-B irradiation in the lignin synthesis pathway, we investigated relative DNA contents in epidermal cells, including trichomes, and enzyme activity and gene expression in the phenylpropanoid pathway. UV-B irradiation increased the ploidy level over 15 days, specifically in the epidermal cells surrounding trichomes, but not in the other epidermal cells or trichomes. In epidermal cells surrounding trichomes, UV-B irradiation induced peroxidase (POX) activity from days 7 to 15. In cotyledons, UV-B exposure induced CS-POX1 and CS-POX3 gene expression within 2 days, and it also induced two other enzymes in the phenylpropanoid pathway, sinapyl alcohol dehydrogenase and coniferyl alcohol dehydrogenase, from days 9 to 11. Thus, exposure to UV-B induces expansion, endoreduplication, POX activity, and the accumulation of polyphenolic compounds in epidermal cells surrounding the trichomes of cucumber cotyledons. Because polyphenolic compounds such as lignin absorb UV-B, our data indicate a physiological protective mechanism against UV-B irradiation in cucumber.

• 2010-03-16

著者

• YAMASAKI Seiji

  Laboratory of Plant Physiology, Department of Science Education, Faculty of Education, Fukuoka Unive

• SHIMADA Eri

  Laboratory of Plant Physiology, Department of Science Education, Faculty of Education, Fukuoka Unive

• KUWANO Tomoe

  Laboratory of Plant Physiology, Department of Science Education, Faculty of Education, Fukuoka Unive

• KAWANO Tae

  Laboratory of Plant Physiology, Department of Science Education, Faculty of Education, Fukuoka Unive

• NOGUCHI Naoto

  Laboratory of Plant Physiology, Department of Science Education, Faculty of Education, Fukuoka Unive

• Shimada Eri

  Laboratory Of Plant Physiology Department Of Science Education Faculty Of Education Fukuoka Universi

• Kuwano Tomoe

  Laboratory Of Plant Physiology Department Of Science Education Faculty Of Education Fukuoka Universi

• Noguchi Naoto

  Laboratory Of Plant Physiology Department Of Science Education Faculty Of Education Fukuoka Universi

• Yamasaki Seiji

  Laboratory Of Plant Breeding And Genetics Department Of Applied Plant Science Graduate School Of Agr

• Yamasaki Seiji

  Laboratory Of Plant Physiology Department Of Science Education Faculty Of Education Fukuoka Universi

• Kawano Tae

  Laboratory Of Plant Physiology Department Of Science Education Faculty Of Education Fukuoka Universi

• Yamasaki Seiji

  Laboratory Of Microbiology And Infectious Diseases Institute Of Scientific And Industrial Research Osaka University

関連論文

• Continuous UV-B Irradiation Induces Endoreduplication and Peroxidase Activity in Epidermal Cells Surrounding Trichomes on Cucumber Cotyledons
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• Continuous UV-B Irradiation Induces Morphological Changes and the Accumulation of Polyphenolic Compounds on the Surface of Cucumber Cotyledons
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