Evidence for a Specific Intracellular Localization of an Antisense Oligonucleotide in K562 Cells

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概要

• 論文の詳細を見る

• In order to be active, antisense oligonucleotides (ASOs) should be delivered to the nuclei of cells. The lack of effect of some ASOs might be explained by poor distribution inside the cell. Here we describe the study of the intracellular distribution of an ASO in a leukemic cell line in which the ASO was not showing an effect. We used fluorescein isothiocyanate-labeled ASO and fluorescent or confocal microscopy. The internalised ASO was localized in a specific intracellular juxtanuclear region, showing no cytoplasmic or nuclear diffusion. Transfection of the ASO improved cellular distribution to the cytoplasm and nuclei and improved the ASO effect.

• 社団法人 日本薬理学会の論文
• 2005-09-20

著者

• Sousa Cristina

  Ipatimup-institute Of Molecular Pathology And Immunology University Of Porto

• Guimaraes Jose

  Ipatimup-institute Of Molecular Pathology And Immunology University Of Porto

• Maia Luis

  Ipatimup-institute Of Molecular Pathology And Immunology University Of Porto

• VASCONCELOS M.

  IPATIMUP-Institute of Molecular Pathology and Immunology, University of Porto

• BELEZA Sandra

  IPATIMUP-Institute of Molecular Pathology and Immunology, University of Porto

• Beleza Sandra

  Ipatimup-institute Of Molecular Pathology And Immunology University Of Porto

• Vasconcelos M.

  Ipatimup-institute Of Molecular Pathology And Immunology University Of Porto

関連論文

• Evidence for a Specific Intracellular Localization of an Antisense Oligonucleotide in K562 Cells

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