Morphological and molecular studies on Sri Lankan Leishmania
スポンサーリンク
概要
- 論文の詳細を見る
Cutaneous leishmaniasis (CL) is an emerging disease in Sri Lanka, more than 400 cases having been reported since 2001. However, the morphology and taxonomic status of the Sri Lankan strain of Leishmania is not known yet. Therefore, it is important to study the morphology and to analyze the phylogenetic position to predict the risk and expansion of the disease and thereby to develop an effective control programme. Morphology of the amastigote of the Sri Lankan isolate was checked by light microscopy and electron microscopic observation. Presence of amastigotes within macrophages was confirmed in skin biopsy samples. The promastigote had the characteristic appearance of a kinetoplastid cell in cultures. The kinetoplast minicircle DNA has been used for diagnosis of Leishmania for a long time and also for phylogenetic studies on trypanosomatid flagellates. The kinetoplast minicircle was amplified using PCR and subsequently sequenced from samples obtained from Sri Lankan patients with cutaneous lesions. Mitochondrial cytochrome b gene has been recently shown to be useful for identification and phylogenetic analysis of the genus Leishmania. The nucleotide sequence of the cytochrome b gene of Sri Lankan Leishmania was determined using the semi-nested PCR and 620 bp of this gene obtained. Phylogenetic analysis using these sequences unambiguously indicated that Sri Lankan isolate of Leishmania belongs to L. donovani complex. However, the Sri Lankan isolate forms a distinct lineage within the complex and probably represents a new branch.
- 2008-12-01
著者
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Le Thanh
Laboratory Of Molecular Parasitology Department Of Immunology Institute Of Biotechnology
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WATANABE Yoshiya
Sophy Co., Research and Development Division
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AGATSUMA Takeshi
Department of Environmental Health Science, Kochi Medical School
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Le Thanh
Institute Of Biotechnology Imunology Department
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RAJAPAKSE R.
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University
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WATANABE Yoshiya
Research and Development Division, Sophy Co.
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Rajapakse R.
Department Of Veterinary Pathobiology Faculty Of Veterinary Medicine And Animal Science University O
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YATAWARA Lalani
Department of Environmental Health Sciences, Kochi Medical School
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WICKRAMASINGHE Susiji
Department of Environmental Health Sciences, Kochi Medical School
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SIYAMBALAGODA R.
Anti-Malaria Campaign, Ministry of Health
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Yatawara Lalani
Department Of Environmental Health Sciences Kochi Medical School
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Agatsuma Takeshi
Department Of Environmental Health Science Kochi Medical School
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Agatsuma Takeshi
Department Of Environmental Health Science Faculty Of Medicine Kochi Medical School
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Watanabe Yoshiya
Sophy Co. Research And Development Division
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Wickramasinghe Susiji
Department Of Environmental Health Sciences Kochi Medical School
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Rajapakse R.
Department Of Veterinary Pathobiology Faculty Of Veterinary Medicine And Animal Science University O
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Siyambalagoda R.
Anti-malaria Campaign Ministry Of Health
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Jayantha Rajapakse
Department of Veterinary Pathobiology, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya
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Laxman R.
Anti-Malaria Campaign, Ministry of Health
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