Optimal Concentration of Calcium and Electric Field Levels Improve Tetraploid Embryo Production by Electrofusion in Mice
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概要
- 論文の詳細を見る
Polyploid embryo production is an important technique in generating mice directly from embryonic stem (ES) cells. The present study was designed to assess the effect of different calcium concentrations and electric field intensities on the production of tetraploid embryos with higher developmental potential by electrofusion. Two-cell mouse embryos were electrofused in fusion solution containing different concentrations of calcium ion (0.05, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4 mM). The rates of blastomere fusion, and subsequent cleavage and development of tetraploids to the blastocyst stage were highest when two-cell embryos were electrically stimulated in a fusion medium containing 1.0 mM calcium. Therefore, we tested electric field intensities (0.6, 0.8, 1.0, 1.2 and 1.4 kV/cm) for electrofusion of two-cell embryos and subsequent development to the blastocyst stage in 1.0 mM calcium. The highest rates of fusion and blastocyst formation were observed when the electric field strength was 0.8 kV/cm. The present results showed that mouse two-cell embryos stimulated with 0.8 kV/cm in a fusion medium containing 1.0 mM calcium had the highest rates of fusion and development to the blastocyst stage.
- 2009-08-01
著者
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Zhu Shi-en
College Of Animal Science And Technology And State Key Laboratory For Agrobiotechnology China Agricu
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Shi Jian-min
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
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Liu Guo-shi
College Of Animal Science And Technology And State Key Laboratory For Agrobiotechnology China Agricu
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Tian Jian-hui
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
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Zeng Shen-ming
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
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SUO Lun
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricu
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WANG Fen
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricu
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ZHOU Guang-Bin
State Key Laboratories for Agrobiotechnology, China Agricultural University
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WANG Yong-Bin
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricu
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ZHU Shi-En
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricu
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LIU Guo-Shi
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricu
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Zhou Guang-bin
College Of Animal Science And Technology And State Key Laboratory For Agrobiotechnology China Agricu
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Suo Lun
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
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Wang Yong-bin
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
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Wang Fen
Laboratory Of Animal Embryonic Biotechnology College Of Animal Science And Technology China Agricult
関連論文
- Optimal Concentration of Calcium and Electric Field Levels Improve Tetraploid Embryo Production by Electrofusion in Mice
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