Divergent roles of tumor suppressor gene Klf6 in ES cell differentiation
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概要
- 論文の詳細を見る
Klf6 is a zinc finger transcription factor that has been identified as a tumor suppressor gene inactivated in several human cancers. We previously reported that Klf6-/- mice are embryonic lethal because of impaired hematopoietic differentiation. In that study, we also found Klf6-/- mice have undefinable livers. Combined with this observation and its developmental expression pattern, Klf6 might have a role in in differentiation of non-hematopoietictissues. The aim of this study was to more clearly define the roles of Klf6 in tissue specification using tissue-specific ES cell differentiation systems. We have evaluated three different culture conditions to induce ES cell differentiation, comparing the responses between Klf6+/+ and Klf6-/- ES cells. Neuronal differentiation of Klf6-/- ES cells was impaired based on marker gene expression and morphology. Interestingly, Klf6 -/- ES cells began to express Hnf3β mRNA, which identified these cells as endodermal precursors, suggesting that Klf6 ordinarily suppresses endoderm differentiation in this culture condition. Additionally, when Klf6-/- ES cells were grown in StemPro34 serum free medium, they showed enhanced cardiac differentiation to a greater extent than Klf6+/+ ES cells, suggesting that Klf6 may have an inhibitory activity in preventing cardiomyogenesis. These findings indicate divergent and tissue- specific roles of Klf6 in ES cell differentiation.
- 日本電気泳動学会の論文
- 2008-12-01
著者
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MATSUMOTO Nobuyuki
Division of Gastroenterology and Hepatology, Department of Internal Medicine, St Marianna University
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ITOH Fumio
Division of Gastroenterology and Hepatology, Department of Internal Medicine, St Marianna University
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Friedman Scott
Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine
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Itoh Fumio
聖マリアンナ医科大学 消化器肝臓内科
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Friedman Scott
Division Of Liver Diseases Department Of Medicine Mount Sinai School Of Medicine
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Zhao Xiao
Division Of Liver Diseases Department Of Medicine Mount Sinai School Of Medicine
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Itoh Fumio
Division Of Gastroenterology And Hepatology Department Of Internal Medicine St Marianna University S
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Matsumoto Nobuyuki
Division Of Gastroenterology And Hepatology Department Of Internal Medicine St Marianna University S
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Matsumoto Nobuyuki
Division Of Liver Diseases Department Of Medicine Mount Sinai School Of Medicine
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