クルマエビにおけるPRDVに対するDNAワクチン接種後の自然免疫関連遺伝子の発現解析
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概要
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In this study, we have developed a DNA vaccine encoding viral envelope protein VP28 of penaeid rod-shaped DNA virus (PRDV, = WSSV). Protective efficacy of the DNA vaccine against PRDV was confirmed at 7 days post vaccination in kuruma shrimp Penaeus japonicus. The VP28 transcript derived from the vaccine was detected in various tissues at 1, 3 and 7 days post vaccination. Moreover, notable up-regulated expression of Rab7, penaeidin, lysozyme and crustin genes was observed upon DNA vaccination. These results suggest that the DNA vaccine significantly increased the protection and innate immune responses in kuruma shrimp against PRDV.
- 2009-06-15
著者
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KONO Tomoya
Faculty of Agriculture, University of Miyazaki
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SONODA Kohei
Faculty of Agriculture, University of Miyazaki
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KITAO Yoichi
Faculty of Agriculture, University of Miyazaki
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MEKATA Tohru
Interdisciplinary Graduate School of Agriculture and Engineering, University of Miyazaki
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ITAMI Toshiaki
Faculty of Agriculture, University of Miyazaki
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SAKAI Masahiro
Faculty of Agriculture, University of Miyazaki
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河野 智哉
Faculty of Agriculture, Miyazaki University
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Kono Tomoya
Faculty Of Agriculture University Of Miyazaki
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Sonoda Kohei
Faculty Of Agriculture University Of Miyazaki
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Mekata Tohru
Interdisciplinary Graduate School Of Agriculture And Engineering University Of Miyazaki
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Kitao Yoichi
Faculty Of Agriculture University Of Miyazaki
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Itami Toshiaki
Faculty Of Agriculture University Of Miyazaki
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Sakai Masahiro
Faculty Of Agriculture University Of Miyazaki
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Sakai Masahiro
Faculty Of Agriculture Miyazaki University
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北尾 陽一
Faculty Of Agriculture University Of Miyazaki
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園田 航平
Faculty Of Agriculture University Of Miyazaki
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