Cell shrinkage of Isochrysis galbana (Prymneshiophyceae) during storage with preservatives

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概要

• 論文の詳細を見る

• Cell volume of Isochrysis galbana (Prymneshiophyceae) was determined with a microscope before and after fixation during storage at 4 and 25°C. The shrinkage effect at 4°C was not detectable but at 25°C. It was described by the exponential equation; Y=0.797+0.224 exp (−0.074 X) for 2% formaldehyde and Y=0.849+0.150 exp (−0.87 X) for 1.6% Lugols solution where X is hours and Y is the ratio of fixed volume to live volume. Lugols solution provides 6.5% less shrinkage than formaldehyde (p<0.05). We recommend Lugols solution as a preservative at 25°C with a shrinkage correction or refrigeration for storage at 4°C, otherwise a species–specific correction should be made for estimating live volume from fixed material.

• 日本プランクトン学会、日本ベントス学会の論文
• 2009-08-01

著者

• Taguchi Satoru

  Department Of Environmental Engineering For Symbiosis Faculty Of Engineering Soka University

• Taguchi Satoru

  Department Of Bioengineering Faculty Of Engineering Soka University

• Iwasawa Kazuo

  Department Of Environmental Engineering For Symbiosis Faculty Of Engineering Soka University

• MURATA AI

  Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University

• Murata Ai

  Soka Univ. Tokyo Jpn

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