Optimization of Medium Components for Manganese Peroxidase Production by White Rot Fungus Strain L-25

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概要

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• The effect of medium component on manganese peroxidase production by white rot fungus strain L-25 was investigated using a response surface method (RSM). The Mn2+ and Polypepton concentrations had a strong influence compared to the initial pH. The Mn2+ concentration affected the specific activity, while the addition of Polypepton elevated the extracellular protein concentration in the medium. Since the MnP activity depended on both the specific activity and extracellular protein concentration, the MnP production could be expressed as a function of both the Mn2+ and Polypepton concentrations. Based on the response surface plot, the optimum medium components were predicted as 1.0 mM Mn2+, 45 g/L Polypepton and initial pH of 6.2. Large amounts of MnP (about 30 U/mL) were obtained using the optimized medium. This is the highest value reported up to now.

• 2008-08-01

著者

• Sakurai Akihiko

  University Of Fukui

• Sakakibara Mikio

  University Of Fukui

• Fujihara Shinya

  University of Fukui

• FUJIHARA Shinya

  Graduate School of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui

• SAKURAI Akihiko

  Graduate School of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui

• SAKAKIBARA Mikio

  Graduate School of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Fukui

関連論文

• Optimization of Medium Components for Manganese Peroxidase Production by White Rot Fungus Strain L-25
• FM-P22 Expression analysis of manganese peroxidase gene in white rot fungus L-25 in the presence of amino acids(Section VIII Fermentation and Microbial Technology)
• FE-P2 Structural features and immuno-stimulating effect of the polysaccharide fractions from Cordyceps militaris(Section IX Food Engineering)
• Removal of Bisphenol A by Peroxidase-Catalyzed Reaction Using Culture Broth of Coprinus cinereus

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