Trf4 is a Useful Gene for Discrimination of Candida tropicalis from other Medically Important Candida Species

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概要

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• Comparative studies of random amplified polymorphic DNA (RAPD) band patterns of Candida tropicalis with those of clinically important Candida species have shown the presence of specific RAPD bands for C. tropicalis. A band specific to C. tropicalis strains (ca. 400 bp) was extracted and sequenced. It was found to belong to a fragment of the Trf4 gene, which is essential for growth of these strains and has a characteristic sequence of C. tropicalis. A PCR primer was designed specifically for C. tropicalis which amplifies the 324 bp band. The PCR primer amplified DNA products for all C. tropicalis strains tested, but did not amplify any PCR bands from C. albicans, C. dubliniensis, C. glabrata, C. guilliermondii, C. kefer, C. krusei, C. parapsilosis, or C. zeylanoides. Usefulness of the PCR primer in differentiating from clinical isolates of other fungal species is discussed.

• 2008-01-31

著者

• Tanaka Reiko

  Medical Mycology Research Center, Chiba University

• MIKAMI Yuzuru

  Medical Mycology Research Center (MMRC), Chiba University

• Tanaka Reiko

  Medical Mycology Research Center Chiba University

• Iida Soji

  Medical Mycology Research Center (mmrc) Chiba University

• Mikami Yuzuru

  Medical Mycology Research Center (mmrc) Chiba University

• Mikami Yuzuru

  Chiba Univ. Chiba Jpn

• KANG Yingqian

  Medical Mycology Research Center (MMRC), Chiba University

• YAMAMOTO Setsuya

  Medical Mycology Research Center (MMRC), Chiba University

• KOGURE Takahisa

  Medical Mycology Research Center (MMRC), Chiba University

• Tanaka Reiko

  Medical Mycology Research Center (mmrc) Chiba University

• Kogure Takahisa

  Medical Mycology Research Center (mmrc) Chiba University

• Kang Yingqian

  Dep. Of Microbiology Guiyang Medical Coll.

• Kang Yingqian

  Medical Mycology Research Center (mmrc) Chiba University

• Mikami Yuzuru

  Medical Mycology Res. Center (mmrc) Chiba Univ.

• Tanaka Reiko

  Medical Mycology Res. Center Chiba Univ.

• Yamamoto Setsuya

  Medical Mycology Research Center (mmrc) Chiba University

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