Stimulation effects of salts on growth in suspension culture of a mangrove plant, Sonneratia alba, compared with another mangrove, Bruguiera sexangula and non-mangrove tobacco BY-2 cells

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概要

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• Effects of a wide range of concentrations of four salts (NaCl, KCl, MgCl2, CaCl2) on cell proliferation were investigated in suspension culture of a mangrove plant, Sonneratia alba. The results were compared to those obtained with suspension culture of another mangrove species, Bruguiera sexangula and non-mangrove tobacco BY-2 cells. Effects of salts on suspension cells were determined with both large scale (LS) method using a 100 ml culture flask, and/or a small scale (SS) method using a 24-well culture plate. Settled cell volume (SCV), packed cell volume (PCV), wet weight (WW) and dry weight (DW) were measured in LS method and PCV was in SS method. High concentrations of NaCl (25–100 mM) and MgCl2 (25 mM) showed strong stimulatory effects on growth in S. alba suspension culture, and low concentrations of KCl, MgCl2 and CaCl2 (10 mM) also showed similar stimulatory effects. In contrast, a remarkable stimulatory effect was not observed in B. sexangula and tobacco BY-2 cells. Cationic ions are effective for the growth of suspension cells in both mangrove species S. alba and B. sexangula. The SS method using multi-well culture plate and micro-tube measurement method developed in this report, can be applied for analysis of factors for stress tolerance.

• 日本植物細胞分子生物学会の論文
• 2008-03-01

著者

• Sasamoto Hamako

  Faculty Of Environment And Information Sciences Yokohama National University

• KAWANA Yoshifumi

  Graduate School of Environment and Information Sciences, Yokohama National University

• Kawana Yoshifumi

  Graduate School Of Environment And Information Sciences Yokohama National Univ.

関連論文

• Stimulatory effects of sea salts on cell growth in liquid culture of Avicenniaceae mangrove
• DEVELOPMENT OF SIMPLE BIOASSAY SYSTEM OF ALLELOPATHY USING PROTOPLAST CULTURES : (MUCUNA PRURIENS AND LETTUCE ROOT
• ENDOGENOUS LEVELS OF PLANT HORMONES IN PROTOPLASTS OF SUSPENSION CULTURE CELLS AND COTYLEDONS OF A MANGROVE PLANT : SONNERATIA ALBA
• Different effects of four salts and pHS on protoplast cultures of a mangrove, Bruguiera sexangula suspension cells, Populus alba leaves and tobacco BY-2 cells
• Stimulation effects of salts on growth in suspension culture of a mangrove plant, Sonneratia alba, compared with another mangrove, Bruguiera sexangula and non-mangrove tobacco BY-2 cells
• Histological analysis in shoot organogenesis from hypocotyl explants of Kandelia candel (Rhizophoraceae)

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