The role of alternating culture temperatures and maltose in enhancing the anther culture efficiency of salt tolerant indica rice (Oryza sativa L.) cultivars, Pokkali and Nona Bokra
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概要
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The effects of different culture temperatures, culture media and saccharides on the anther culture efficiency in two salt tolerant rice (Oryza sativa ssp. indica) cultivars, Pokkali and Nona Bokra, were examined. Comparison of two culture temperatures (constant temperature at 25°C and alternating temperatures at 30°C/20°C) indicated that alternating temperatures showed remarkable effects not only on the callus induction but also on the plant regeneration. Among the basal media examined, higher callus productivity was observed in SK-I medium and higher shoot productivity was obtained in SK-II medium than other basal media. When alternating temperatures and revised N6 medium including maltose were used throughout anther culture, callus productivity and green shoot productivity showed the highest score in the salt tolerant two cultivars used in this study. Maltose and alternating temperatures increases the anther culture efficiency of recalcitrant indica rice cultivars. This is the first report suggesting high production of haploids or doubled haploids from anther culture of these salt tolerant indica cultivars.
- 2007-06-01
著者
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Kamijima Osamu
Faculty Of Agriculture Kobe University
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Misoo Shuji
Faculty Of Agriculture Kobe University
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JAVED Muhammad
Graduate School of Science and Technology, Kobe University
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ISHII Takashige
Faculty of Agriculture, Kobe University
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Javed Muhammad
Graduate School Of Science And Technology Kobe University
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Ishii Takashige
Faculty Of Agriculture Kobe University
関連論文
- The role of alternating culture temperatures and maltose in enhancing the anther culture efficiency of salt tolerant indica rice (Oryza sativa L.) cultivars, Pokkali and Nona Bokra
- Mechanism of High Photosynthetic Capacity in BC_2F_4 Lines Derived from a Cross between Oryza sativa and Wild Relatives O. rufipogon(Crop Physiology and Ecology)
- Enhancement of Rice Leaf Photosynthesis by Crossing between Cultivated Rice, Oryza sativa and Wild Rice Species, Oryza rufipogon(Crop Physiology and Ecology)