The tobacco CHN50 matrix attachment region enhances transformation frequency

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概要

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• The nuclear matrix attachment region (MAR) located upstream from the tobacco gene CHN50, designated CHN50MAR, consists of two MAR elements: S/M I and S/M II. In a direct gene transfer system using tobacco BY-2 cells, CHN50MAR produced a several-fold increase in colony formation of hygromycin-resistant (hygR) cells when arranged adjacent to both the 5′ and 3′ termini of a hygromycin-selective marker gene. S/M I and S/M II enhanced hygR colony formation to the same degree as CHN50MAR, suggesting that these MAR elements are functionally redundant for enhancing transformation. The presence of the marker genes in the hygR cells was confirmed using the polymerase chain reaction. These results imply that CHN50MAR and its MAR elements are available as transformation enhancers in direct gene transfer systems for plants.

• 日本植物細胞分子生物学会の論文
• 2006-12-01

著者

• Fukuda Yuji

  International Patent Organism Depositary National Institute Of Advanced Industrial Science And Techn

• SHIRAI Kikuko

  International Patent Organism Depositary, National Institute of Advanced Industrial Science and Tech

• Shirai Kikuko

  International Patent Organism Depositary National Institute Of Advanced Industrial Science And Techn

関連論文

• The tobacco CHN50 matrix attachment region enhances transformation frequency

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