初期胚神経上皮由来神経幹細胞の脳移植ドナーとしての可能性に関する検討
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概要
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原著The biological character of neuroepithelial stem cells (NESCs) was assessed ix vitro and in vivo to confirm the feasibility of using them as donor cells for intracerebral grafting. All of the NESCs, which were derived from mesencephalic neural plates, were immunohislochemically positive for both nestin and fibroblast growth factor〔FGF) receptor. Isolation and proliferaUon of NESCs was attempted from culture under various conditions. Neurospheres fomed in medium conしaining FGF2, and NESCs were able to grow at a maximum proliferation rate of approximately 7.3 fold in seven days. The single cells derived from primary spheres differentiated into neurons that extended long neurites in two days and expressed several neurochemical markers, suggesting maturation of the cells, but hardly any filial cells were observed. The growth potential of the NESCs, however, subsequently diminished even in the medium containing FGF2, and NESCs derived from FGF2-responsive neurospheres after serial weekly passages tended to differentiate into glial cells more than into eurons. The NESCs proliferated in seven days with FGF2, which was ransplanted into normal rat striata, also expressed nestin and TuJ I, but GFAP positive cells were hardly seen. These experimental results indicated that the neural stem cells were not always equal in their differ entiation paths and that the features of neural stem cells could be changed with the duration of culture.NESCs could be proliferated with the addition of FGF2 with having preserved vigorous neuronal differennation depending on the required amount at its maximum under FGF2 at the 7-day time point, suggesting that 7-day-old NESCs proliferated with FGF2 may be favorable donor cells for inlracerebral grafting torestore damaged neuronal circuits in diseased brain.
- 慶應医学会の論文
- 2007-06-25