ループレス結晶マウント法の開発とCr Kα線による非標識タンパク質の結晶構造解析

概要

論文の詳細を見る
A novel crystal mounting technique was developed for the sulfur SAD(S-SAD)method using longer wavelength X-rays. With this novel technique, the cryo-buffer and cryoloop surrounding the protein crystal can be removed before data collection to eliminate their X-ray absorption. Structures of several novel proteins have been solved by S-SAD using this mounting technique in combination with in-house chromium Kα radiation(2.29 Å). Our novel crystal mounting technique is superior to the standard cryoloop mounting method for the measurement of small anomalous differences, and yields good results in sulfur substructure solution and phasing by the SAD method. These results indicate that the S-SAD method with a chromium source becomes more practical for macromolecular structure determination using our crystal mounting technique.
2006-08-30