Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton
スポンサーリンク
概要
- 論文の詳細を見る
We evaluated the suitability of ELF-based assay (ELF: Enzyme-Labeled Fluorescence, Molecular Probes) as a tool for specific-detection of alkaline phosphatase (AP) activity in individual species of marine phytoplankton. Among 4 species of marine phytoplankton tested in the present culture experiments, the ELF-labeled cells of Karenia mikimotoi (Dinophyceae) and Skeletonema costatum (Bacillariophyceae) were simultaneously found with AP activity that was determined by using 4-methylumbelliferyl phosphate under the phosphorus-starved state but not found under the phosphorus-replete state. There were significant positive correlations between the percentage of ELF-labeled cells and AP activity of K. mikimotoi and of S. costatum. In contrast for Chattonella antiqua and Heterosigma akashiwo (Raphidophyceae) which do not produce AP, no ELF-labeled cells were found even under the phosphorus-starved state. Thus, ELF-based assay is considered to be useful for specific-detection of AP activity.
- 日本プランクトン学会の論文
- 2006-11-01
著者
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YAMAGUCHI Mineo
Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisherie
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ADACHI MASAO
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University
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Adachi Masao
Laboratory Of Aquatic Environmental Science Department Of Aquaculture Faculty Of Agriculture Kochi U
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YAMAGUCHI Haruo
Laboratory of Aquatic Environmental Science, Faculty of Agriculture, Kochi University
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Yamaguchi Haruo
Kochi Univ. Kochi Jpn
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Adachi Masao
Kochi Univ. Kochi Jpn
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YAMAGUCHI HARUO
Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisherie
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Yamaguchi Mineo
Harmful Phytoplankton Section Harmful Algal Bloom Division National Research Institute Of Fisheries
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Yamaguchi Mineo
Harmful Algal Bloom Division National Research Institute Of Fisheries And Environment Of Inland Sea
関連論文
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