Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR

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概要

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• We designed a new inverse PCR protocol combined with switching mechanism at 5′ end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5′- and 3′-ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.

• Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
• 2006-08-23

著者

• Sakai Yoichi

  Graduate School Of Biosphere Science Hiroshima University

• Kuniyoshi Hisato

  Graduate School Of Biosphere Science Hiroshima University

• FUKUI Yukio

  Graduate School of Biosphere Science, Hiroshima University

• Fukui Yukio

  Graduate School Of Biosphere Science Hiroshima University

• Kuniyoshi Hisato

  Graduate School of Biosphere Science, Hiroshima University

関連論文

• Cloning of full-length cDNA of teleost corticotropin-releasing hormone precursor by improved inverse PCR
• Molecular Cloning and Expression Profile of Sex-specific Genes, Figla and Dmrt1, in the Protogynous Hermaphroditic Fish, Halichoeres poecilopterus
• Ovarian degeneration during the female-to-male sex change in the protogynous hermaphroditic fish, Halichoeres poecilopterus

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